Reporter

Part:BBa_K812130:Design

Designed by: Anna Mlynarczyk   Group: iGEM12_Evry   (2012-09-20)


Citrine reporter with a Kozak sequence for expression in Xenopus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Kozak is included, as it cannot be added by biobrick. This part is ready for direct injection into a fertilised Xenopus tropicalis egg.


Source

Original sequence from A. victoria mutated to emit yellow light

References